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N. Ali, Ekhlass
- Immunomodulatory Effects of Pediococcus Acidilactici on Balb/C Mice Immunosuppressed by Cyclophosphamide
Authors
1 Biology Department, College of Science, Mustansiriyah University,, IQ
Source
Research Journal of Pharmacy and Technology, Vol 15, No 2 (2022), Pagination: 605-610Abstract
Background: The live microorganisms that present in food in addition dietary supplements called Probiotics they have beneficial effect in the human intestine. In the last years, Probiotics has become within treatment options for several disease included immune system. The advent and use of probiotics seem to be increasing day by day all over the world. Objective: The current study included detection of efficacy Pediococcus acidilactici as immunomodulatory in mice that treated with cyclophosphamide. Design: To detect efficacy of Pediococcus acidilactici as immunosuppressive model was used. This model was completed by taking 20 male BALB/C mice with six-weeks old to divide into (5) groups :(G 1) group is the normal control. (G2) group: the group that injected with cyclophosphamide. (G3) group: the immunosuppression plus Pediococcus acidilactici (6×108 CFU/ml). (G4) group: the immunosuppression plus Pediococcus acidilactici (6×104 CFU/ml). last (G5) group : is the group that treated with probiotics. Results: In the current study, thymus and spleen indicators were significantly higher in treated groups than those of (G1) group (NC) with (0.323±0.34) (P<0.05), also Macrophages phagocytosis showed a clear increase in the three treated groups (76.625±108, 72.125±1.65, 87.750±1.32) respectively with significantly (P<0.05), as a compared with (G1) group (NC). Conclusions: The current study reveals the ability of Pediococcus acidilactici (6×109) CFU/ml to accelerate the healing of cyclophosphamide immunosuppressive mice. So, the activity role of probiotic strain as immunomodulator in this study leads us to urge the use it as an alternative treatment for chemical drugs that used against immunosuppression.
Keywords
P.acidilactici ; Spleen and thymocyte index; Macrophages phagocytosis. INTRODUCTION: Probiotics can be defined that live microorganisms have many beneficial effects to human body, they can be isolated from many sources like food, so they are considered asReferences
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- Preparation and Analysis of Faba Bean Pods Agar with Identification of C. Neoformans
Authors
1 Department of Biology, College of Science, Mustansiriyah University, Baghdad,, IQ
2 College of Basic Education, Wasit University, Baghdad, IQ
Source
Research Journal of Pharmacy and Technology, Vol 15, No 3 (2022), Pagination: 1097-1100Abstract
Background: Recently researchers used agar that prepared from natural products and can be used in biological labs to grow bacteria and fungi such as C. neoformans. Objective: This study aimed to prepare a cultural media agar based on extract from pods of fava bean and use it to cultured and identify C. neoformans and compare it with negative control (C. albicans). Materials and Methods:C. neoformans and C. albicans isolates were obtained from laboratory of Biology Department, College of Science, University of Mustansyria, and then the pods were grinded, dissolved in sterile distilled water, boiled for 10 min. The extract was then filtered and sterilized in autoclave, then cooled and poured into sterile Petri dishes. C. albicans and C. neoformans were incubated for 48 hours. Also, and after treating the extract with methanol, and the HPLC was taken to investigate its components. Results: C. neoformans showed dark brown colonies, while C. albicans showed white colonies, and the HPLC showed that the fava bean pods content acids such as Ascorbic, Gallic, Caffeic, and Methyl gallat. Conclusions: Fava bean pods agar used to identify C. neoformans based on producing phenoloxidase enzyme which oxidizes phenolic compounds in the medium.Keywords
C. neoformans, Melanin pigment, Vicia fabapods, Iraq.References
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